For Determining Areas of
Related to Laboratory Self-inspection Forms
The following guide
has been developed to assist you in your scheduled safety surveillance
of laboratories and departments under your auspices as safety coordinator.
This guide is by no means all encompassing, however information contained
after each item should assist you in determining whether your area may
be in full, partial or non-compliance.
Keep in mind that
all Federal, State and University rules, recommendations and regulations
determine the compliance of our area concerning OSHA, EPA, NIH, CDC, and
DOT. If you have any specific questions on the information below, please
turn to the end of these guidelines for the names and numbers of individuals
at the University who will be able to assist you. Updates will be distributed
to safety coordinators as they occur.
- Entrances, Exits,
Hallways and Stairways - All entrances, exits, hallways and stairways
must be clear and unobstructed.
- Showers/Eye Operative
- Monthly inspections are required. Any area which deals with corrosive,
flammable or otherwise hazardous material is required to have immediate
access to eyewash and drench shower facilities. All showers and eye
wash equipment must be in full operational order and unobstructed. Eye
wash bottles are not adequate equipment.
- Personal Protective
Equipment - Personal Protective Equipment such as goggles, masks, gloves
and cover gowns must be readily available and not worn outside the immediate
work areas. Lab coats and appropriate shoes shall be worn to avoid any
contact with harmful materials. Respirators shall be used when applicable.
Evidence of respirator training and certification must be readily available.
- Fire Extinguisher/Inspection
and Location - All fire extinguishers must be inspected monthly. Extinguishers
must be properly mounted, unobstructed and be properly labeled for the
intended use. Training classes are offered through the UK Fire Marshal.
- Pressurized Cylinders
- All cylinders must be stored in proper locations. All cylinders must
be secured in an upright position and properly restrained to prevent
falling. Containers must be labeled for contents and usage. Cylinders
of all gases having a Health Hazard Rating of 3 or 4 shall be kept in
a continuously mechanical ventilated hood or other ventilated enclosure.
Maximum number of cylinders of a flammable gas shall be not more than
3 (10" x 50") per 500 square feet in an unsprinkled space or not more
than 6 (10" x 50") in a sprinkled space of 500 square feet.
Liquefied Gas Cylinders
in laboratory work areas shall not exceed 3 cylinders (9" x 30") in
a sprinkled space or exceed 2 cylinders (9" x 30") in an unsprinkled
- Room Use Identification
- All access doors must be marked when rooms or areas are being used
for chemical, biological or radioactive purposes as outlined in the
UK Chemical Hygiene Plan. All doors must remain closed and the vision
panel must remain unobstructed. Unattended labs shall be locked at all
- UL Electrical
Equipment and Cords - Only Underwriters Laboratories approved equipment
and cords are authorized for use. Only UL listed mullet outlet strips
equipped with 15 AMP circuit breakers are approved.
- Fume Hood Operation
- Face Velocities should be between 80 and 150 FPM at the working sash
height with an optimum level of 100 FPM. The sash should never be higher
than 12 inches except when accessing equipment. Hoods should
not be located in high traffic areas or under air supply vents. The
hood must have user spill protection and cup sinks must have spill guards.
- Biological Safety
Cabinets - Certification is required annually or any time the hood is
moved or has had maintenance performed. Cabinets must not be located
near high traffic areas or air supply ducts.
- Hazardous Chemicals
- All chemicals must be appropriately labeled and shall not be placed
near or over floor drains. Flammable liquids must be stored in appropriate
containers. 0-10 gallons must be stored in shipping containers. 11-20
gallons must be stored in UL listed safety containers, 21 and over must
be stored in a safety cabinet.
- Hazardous Waste
Disposal - Hazardous waste training is required for all employees who
handle hazardous material. Training is given by the Office of Hazardous
Material Management (HMM). A certification of training must be posted
in laboratories. Contact HMM for the time and date of classes.
- Equipment and Utility
Labeling - All utility and plumbing lines need to be labeled and indicate
the product contained; i.e., gas, water, etc.
- Location of Cut-off
Valves/Circuit Breakers - All cut off valves and breakers must be properly
- General Safety
(Dress, Eating, Smoking, etc.) - Eating, drinking, smoking and applying
cosmetics is not permitted in a wet lab.
ice machines and microwaves must be labeled for intended use.
- Food, personal
medication and hazardous materials shall not be housed in the same
- Smoking is
prohibited throughout all facilities at the University (with
the exception specially designed "designated smoking areas").
- Lab Personnel
shall not wear loose (e.g. saris, dangling neckties, overly large
or ragged lab coats), skimpy (e.g. shorts and/or halter tops), torn
clothing, or unrestrained long hair. Perforated shoes, sandals,
or cloth sneakers are not to be worn in labs.
- Use of Flame and
Heat - No heat generating devices should be left unattended.
- Ventilation - Air
flow in most labs should be "negative" with respect to the corridor.
Laboratory doors shall be kept closed when laboratory procedures are
in progress. Volatile hazardous materials shall not be used on the open
Flushed - All unnecessary material, boxes, and containers must be disposed
of in the appropriate manner. All drains, including floor drains and
cup sinks should be flushed with water on a weekly basis to eliminate
sewer odors. Proper housekeeping must be maintained to provide adequate
clearance of sprinkler systems and emergency equipment.
- Sharps (Glass,
Scalpel, Blades, Syringes, Etc.) - All sharps, needles and glass must
be disposed of in an approved labeled container. Glass containers and
other potentially sharp objects shall not be disposed of in common office
refuse. Containers must not be overfilled and must be labeled and sealed
for proper handling and disposal.
- Emergency lighting
- Where necessary, emergency lighting units shall be properly mounted
and obstructed. If emergency lighting exists, it should be checked periodically
to ensure it is functional.
- Emergency Plans/Posted
Numbers - All emergency and contingencies plans and evacuation routes
shall be clearly posted in conspicuous places. A list of emergency numbers
and contacts must be kept updated and posted along side the emergency
- Safety Manuals
- Manuals must be current and readily available for all employees.
- Accidents Reported/Investigated
- All accidents must be reported to the immediate supervisor for the
completion of the appropriate form. File copies of reported incidents
and accidents must be on hand, as well as the action taken to alleviate
the safety hazard in the future.
- Safety Training
- This area is designated for either the recommended training which
is required by law.
for Disease Control
Safety Data Sheets
Safety & Health Administration
Institutes of Health
for a copy of the laboratory self inspection form.